Figure 5

MiR-143 targeted oncogene FNDC3B. A, The putative miR-143-binding sequence was in the 3^′-UTR of FNDC3B mRNA. B, The dual luciferase report assay of HEK-293 T cells cotransfected with psi-CHECK2-FNDC3B and miRNA (miR-143, NC). The Renilla luciferase signals were normalized to the internal firefly luciferase transfection control. C, The mRNA and protein expression of FNDC3B in PC-3-M cells transfected with miR-143 inhibitor or NC was analyzed by RT-PCR (above) and western blotting (below). D, The expression of miR-143 and FNDC3B in the tumor tissues of nude mice were analyzed by RT-PCR (above) and western blotting (below). U6 and GAPDH were internal control. (* P < 0.05).