The down-regulation of miR-143 repressed PC-3-M metastasis in vivo. A, The down-regulation of miR-143 in PC-3-M stable cell lines on days 0 and 60 by shRNA was verified by qRT-PCR. B, Systemic metastases of PC-3-M cells was measured in mice by bioluminescence imaging 30 days post-implant. C, The liver metastasis of PC-3-M cells was measured by macroscopic and bioluminescent methods after autopsy. D, Histologic analysis of metastatic lesions in the ribs of nude mice was carried by H&E-staining in which tumors were marked as “T”, bone was “B” and muscle was “M”.