Figure 3

Eugenol suppresses the expression of several oncoproteins. (A) Cells were either sham-treated (DMSO) or challenged with eugenol (2 μM) for 24 hrs. Subsequently, cells were harvested and proteins were used for western blot analysis using the indicated antibodies. The numbers under the bands represent the corresponding expression levels as compared to time 0 and after normalization against GAPDH. (B) DMSO- and eugenol-treated cells (2 μM) were harvested after 4 hrs, and total RNA was extracted and subjected to RT-PCR using specific primers for the indicated genes. The resulting products were electrophorezed in ethidium bromide stained 2% agarose gel. The numbers under the bands represent the corresponding expression levels as compared to control (DMSO) and after normalization against β-actin.