Figure 2
From: Eugenol triggers apoptosis in breast cancer cells through E2F1/survivin down-regulation
Eugenol triggers apoptosis through the mitochondrial pathway. MDA-MB-231 cells were treated with eugenol (2 μM), and then were harvested at the indicated periods of time. Proteins (50 μg) were used for western blot analysis utilizing antibodies against the indicated proteins. The numbers below the bands represent the corresponding expression levels as compared with time 0 and after normalization against GAPDH.