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Table 2 Autocrine loop induced by EREG in U87 cells

From: High epiregulin expression in human U87 glioma cells relies on IRE1α and promotes autocrine growth through EGF receptor

  Division time in days (R value)
  U87Ctrl U87dn
no treatment 2.73 (0.99) 5.08 (1.00)
EREG 2.20 (1.00) 2.86 (0.99)
EREG/Erbitux® 3.08 (1.00) 4.50 (1.00)
anti-EREG 3.12 (1.00) 4.76 (0.99)
  1. Cells were plated at 10 000 cells/cm2 in 24-well plates and grown for four successive passages in serum-free condition in the presence or absence of 10 ng/ml EREG, with or without antibodies anti-ErbB1 (Erbitux®, 200 μg/ml) or anti-EREG (5 μg/ml). Cells were counted at each passage and division times were presented as best slopes obtained after four passages (29 days of growth) and from a series of triplicate experiments. Regression lines include the origin (R, correlation coefficient). Control mouse monoclonal antibody (isotype IgG1) had no significant effect.