High epiregulin expression in human U87 glioma cells relies on IRE1α and promotes autocrine growth through EGF receptor

Table 2 Autocrine loop induced by EREG in U87 cells

Cells were plated at 10 000 cells/cm² in 24-well plates and grown for four successive passages in serum-free condition in the presence or absence of 10 ng/ml EREG, with or without antibodies anti-ErbB1 (Erbitux®, 200 μg/ml) or anti-EREG (5 μg/ml). Cells were counted at each passage and division times were presented as best slopes obtained after four passages (29 days of growth) and from a series of triplicate experiments. Regression lines include the origin (R, correlation coefficient). Control mouse monoclonal antibody (isotype IgG1) had no significant effect.

ISSN: 1471-2407