Silencing of WWOX results in increased clonal growth, decreased attachment and increased cell motility. (A) Western blot demonstrating WWOX silencing in cell extracts from MCF10 control (Scr) and three independent stably WWOX-silenced cell lines. (B) Effect of WWOX-silencing on clonal growth. Five hundred cells were seeded in each well of a 6-well plate for each subline and allowed to grow for 9 days. Representative image of both conditions is shown. Bar graph represents the average of three independent experiments +/- SEM. (C) Scratch wound healing assay. Results of four separate experiments done in biological triplicates (two wounds per well). Depicted is the average percent of the difference between T0 and T24 from all experiments (±SEM).