Figure 3
Effect of various treatments on iNOS expression in glioma cells in vitro and in vivo . (a) U251 and 5310 cells were transfected with scrambled vector (SV-sh), MMP-9 plasmid shRNA (M-sh), uPAR plasmid shRNA (U-sh), or MMP-9 + uPAR plasmid shRNA (MU-sh) and then subjected to immunocytochemical analysis for iNOS expression. (b) Immunohistochemical comparison of the iNOS expression in control, M-sh-, U-sh- and MU-sh-treated nude mice that were pre-injected (intracerebrally) with 5310 cells (0.2 × 106 cells). (c) RT-PCR analysis of U251 and 5310 cells transfected with full-length MMP-9 (M-fl), and uPAR (U-fl) plasmids to evaluate the changes in iNOS mRNA expression.