Figure 2

LF-MF inhibits the proliferation and induce cell cycle arrest in B16-F10 cells. (A) CFSE proliferation assay analyzes the proliferation of B16-F10 after exposed to sham LF-MF (Con) or LF-MF (2 h/day, 5 days); The means of proliferation index using CFSE assay (B); CCK-8 assay (C) and simple cell counting (D) detects the cell proliferation in sham LF-MF (Con) and LF-MF (2 h/day, 5 days). (E) Flow cytometric analysis of cell cycle after exposure to LF-MF for 3, 4 and 5 days. (F) The mean percentage of G1, S and G2 phase cells. All the experiments were repeated three times. Data are expressed as means ± S.E.M.