Figure 3
From: The anti-tumor effect of shikonin on osteosarcoma by inducing RIP1 and RIP3 dependent necroptosis
The induction of necroptosis by shikonin via upregulating RIP1 and RIP3 in K7 and U2OS cells. K7, U2OS and 143B cells were treated with different concentrations of shikonin for 8Â hours. The expression of RIP1, RIP3, caspase-3, caspase-6 and PARP was detected by Western blot. The expression of the necroptosis related proteins, RIP1 and RIP3, was increased while apoptosis related proteins, caspase-3, caspase-6 and PARP were not activated in K7 and U2OS cells after being treated with shikonin. The expression of RIP1 and RIP3 had no obvious changes and caspase-3, caspase-6 and PARP were not activated in 143B cells after being treated with shikonin. Data are representative of 3 independent experiments.