Reduced SEC62 expression correlates with slightly increased sensitivity to TFP in PC3 cells. A, Effect of ophiobolin A treatment on the growth of PC3 and HeLa cells. PC3 or HeLa cells (1 × 104) were seeded in a 96-well ePlate and growth was examined by the xCELLigence RTCA system. After 330 min, cells were treated with buffer alone or buffer + ophiobolin A at the indicated concentrations. All samples were measured in triplicate. The cell index was normalized to the time point of cell treatment (330 min). B, The same analysis as described in A was performed on PC3, HeLa and HEK293 cells after treatment with TFP at the indicated concentrations. C, Quantification of the ER proteins, Sec62 and BiP, by western blot analysis. D, PC3 or HeLa cells were seeded in normal growth medium without FBS and supplemented with ophiobolin A, TFP or DMSO (control) at the indicated concentrations in the top chambers of a BD-Falcon Fluoroblok migration system (24-well format). The upper chambers were set in the lower chambers, which contained the same medium with 10% FBS as an attractant. After 72 h (PC3) or 24 h (HeLa), migrated cells were fixed with methanol and DAPI stained. Migration was analyzed by fluorescence microscopy. The quantitative data from this experiment are shown in the diagram.