Establishment and characterization of the myxofibrosarcoma cell line MUG-Myx1. (A) IHC analysis on patient tumour tissue showed poorly differentiated tumour components (left) in connection with better differentiated tumour areas with myxoid stroma and curvilinear blood vessels (right). (B) The hematoxylin and eosin (H&E) staining of the MUG-Myx1 cell line showed spindle and multinucleated tumour cells. (C) Strong expression of Vimentin of the MUG-Myx1 cell line confirmed the mesenchymal origin of the tumour cells; nuclei were stained with DAPI. (D) Dynamic proliferation curves for MUG-Myx1; 5 × 103 and 1 × 104 cells were seeded per well and measured with the xCELLigence system. (E) MTS proliferation analysis revealed a 24 h doubling time. (F) The DNA index of 1.15 indicated hyperdiploid tumour cells (left). Gating strategy of cell cycle analysis, to exclude doublets from the total population (right). (G) MUG-Myx1 Tumour formation in NOD/SCID/IL-2rγnull (NSG-) mice.