Figure 1

PHD1, PHD2, PHD3 and FIH transcript and protein levels in primary cancerous and histopathologically unchanged tissues from patients with CRC. A. The cancerous (●) and histopathologically unchanged tissues (○) from ninety patients with CRC were used for RNA and protein isolation. Total RNA was reverse-transcribed, and cDNAs were investigated by RQ-PCR relative quantification analysis. The PHD1, PHD2, PHD3 and FIH mRNA levels were corrected by the geometric mean of PBGD and hMRPL19 cDNA levels. The amounts of PHD1, PHD2, PHD3 and FIH mRNA were expressed as the decimal logarithm of multiples of these cDNA copies in the calibrator. B. Proteins were separated by 10% SDS-PAGE, and transferred to a membrane that was then immunoblotted with Rp anti- PHD1, - PHD2, - PHD3 and - FIH Ab and incubated with goat anti-rabbit HRP-conjugated Ab. The membrane was then stripped and blotted with Rp anti-GAPDH Ab, followed by incubation with goat anti-rabbit HRP-conjugated Ab. The amount of western blot-detected PHD1, PHD2, PHD3 and FIH proteins was presented as the decimal logarithm of PHD1, PHD2, PHD3 and FIH to GAPDH band optical density ratio. The p value was evaluated by unpaired, two-tailed t-test or U-Mann-Whitney test.