Expression of Annexin A7 mRNA and protein. (A) RT-PCR analysis of Annexin A7 gene silencing in Hca-F cells using three shRNA constructs (Annexin A25, Annexin A59, and Annexin A507). pSilencer-Annexin A25 shRNA was more potent than pSilencer-Annexin A507 and pSilencer-Annexin A59 shRNA. (B) RT-PCR analysis of Annexin A7 mRNA expression in Hca-P cells. (C) Western blot analysis of Annexin A7 expression in Hca-F, FANXA7-control, and FANXA7-down cells.
(D) Western blot analysis of Annexin A7 expression in Hca-P, PANXA7-control, and PANXA7-up cells.