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Table 2 Real-time RT-PCR was used to confirm Versican expression

From: CD26 Expression on T-Anaplastic Large Cell Lymphoma (ALCL) Line Karpas 299 is associated with increased expression of Versican and MT1-MMP and enhanced adhesion

GAPDH Avg Ct Karpas/Dep1 Karpas/Dep2
Karpas 17.74 - -
Dep1 16.70 0.49 -
Dep2 16.72 - 0.49
CD26    
Karpas 20.93 - -
Dep1 23.95 8.11 -
Dep2 24.05 - 8.69
Versican    
Karpas 25.51 - -
Dep1 31.83 80 -
Dep2 32.20 - 103
  1. RNA was isolated from Karpas 299 cells and two clones, Dep1 and Dep2, in which CD26 is depleted. SYBR Green-based real-time RT-PCR was carried out on 10 ng total RNA using QuantiTect Primer Assays for CD26, Versican, and GAPDH.