Figure 2

Induction of senescence after ectopic expression of SORBS2 in primary fibroblasts and keratinocytes. Beta-galactosidase staining was performed at different time points after lentiviral transduction. Depicted are cells stained 14 days after transduction of pCDH empty vector (control) and SORBS2-2. Only a few senescent cells (arrows) are evident in the control (A). An at least 4-fold increase in senescent cells is observed after transduction of SORBS2 both in primary human fibroblasts and keratinocytes (B). All images were captured at 200× magnification. Expression of the transgene in cells at same passage was confirmed by Western blot (C).