Figure 4

B82 inhibited H460 xenograft tumor growth in athymic mice. One day after injected with H460 cells, once daily i.p. injection of B82 (5 mg/kg) or vehicle was continued for the duration of the experiment. A. The volumes of the tumors (n = 10); B. The mice were sacrificed on day 29 and the tumors were weighed (n = 10). C. The tumor tissues from B82-treated or vehicle control mice were collected, proteins were extracted from tumors, and were subjected to western blot analysis for the determination of CHOP and cleavaged caspase-3 (n = 4). D. Tumor tissues (5 μm section) from each group (n = 4) were processed for immunochemistry analysis for Bcl-2, Cyclin D1, and COX-2. E. The column figure gives a quantitative analysis of relative amount of above proteins in tumor tissues (* p < 0.05, compared with control group).