Figure 6
From: Selenized milk casein in the diet of BALB/c nude mice reduces growth of intramammary MCF-7 tumors
Effect of Se-casein on apoptotic cell number. After 70 d on Se-casein treatment, the 4 largest MCF-7 tumors on each treatment were excised, embedded in wax, and sectioned at 5 μm onto microscope slides.The TUNEL assay was used to identify apoptotic cells and nuclei were counterstained with hematoxylin. a) mouse 13, 0.16 ppm Se. b) mouse 26, 0.51 ppm Se. c) mouse 3, 0.85 ppm Se. d) mouse 15. 1.15 ppm Se. Arrows indicate apoptotic cells. e) Apoptotic cell number was expressed as a percentage of total nuclei, counting 1500–2000 nuclei per sample. Values are means ± SE for each treatment group. The P-value represents the linear effect of dietary Se level.