Figure 6
From: Selective apoptosis induction in MCF-7 cell line by truncated minimal functional region of Apoptin
Detection of Apoptotic MCF7 cells by Annexin V and PI. MCF7 cells that were microinjected with A) PrVP3, B) PrVP3A1-69N1N2, C) PrVP3A1-46N1N2, D) PrVP3A1-31N1N2, E) PrVP3A32-69N1N2, F) PrVP3A32-62N1N2, G) negative control PrMBP and H) non-injected cells were analyzed by Annexin V assay at 5 hr and 15 hr. Green fluorescence signal in the cell showed the presence of apoptotic cells (red arrow), on the other hand the presence of both green and red fluorescence in the cell indicated cell necrosis (yellow arrow). Lesser apoptotic cells were observed at 5 hr than 15 hr.