Figure 5
From: Selective apoptosis induction in MCF-7 cell line by truncated minimal functional region of Apoptin
Indirect immunofluorescence assay on microinjected Chang cells with VP3 protein and truncated VP3 protein. Chang cells were injected with A) PrVP3, B) PrVP3A1-69N1N2, C) PrVP3A1-46N1N2, D) PrVP3A1-31N1N2 E) PrVP3A32-69N1N2, F) PrVP3A32-62N1N2 and negative control, G) PrMBP. The microinjected cells were assayed at 2 hr and 10 hr. At both time points, the injected protein (with green fluorescence) was detected at the cell cytoplasm (shown in red arrow).