Figure 4

Chemotherapy treatment increases ABCC2 expression in ovarian cancer cells. A. qRT-PCR for ABCC2 expression. Ovarian cancer cells treated with LD₅₀ CBP for 72 hr. OVCAR-5 cells also treated in presence or absence of HA oligomers (HYA oligo, 250 μg/ml). Relative gene expression was determined by calibration against no CBP control and normalized to the house keeping gene β-actin using the 2^-∆∆CT method. Data represents mean ± SEM from 3 independent experiments performed in triplicate. B. OVCAR-5 treated with LD₅₀ CBP for 72 hr. Fixed cells were incubated with biotinylated HABP and ABCC2 mouse monoclonal antibody and visualized with Cy3-conjugated streptavidin (red) and FITC-conjugated donkey anti-mouse immunoglobulins (green) respectively. Nuclei are counterstained with DAPI dye (blue).