MicroRNA-21 promotes hepatocellular carcinoma HepG2 cell proliferation through repression of mitogen-activated protein kinase-kinase 3

Table 2 The sequences of primers used for reverse transcription and PCR

Application	Primer	Sequence (5’→3’)
Reverse transcription	miR-21 RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCAACATC
Reverse transcription	U6 RT	AACGCTTCACGAATTTGCGT
qRT-PCR of miR-21	Common primer	CTCAACTGGTGTCGTGGA
qRT-PCR of miR-21	miR-21 PCR	ACACTCCAGCTGGCTAGCTTATCAGACTGATG
qRT-PCR of U6	U6 promoter forward	CTCGCTTCGGCAGCACA
qRT-PCR of U6	U6 promoter reverse	AACGCTTCACGAATTTGCGT

ISSN: 1471-2407