miR-21 expression in HepG2 cells. The expression of miR-21 in HepG2 cells infected with Ad/pri-miR-21, Ad/miR-21/inhibitor or Ad/con virus was determined by a qRT-PCR assay. Significantly increased expression of miR-21 was detected in the cells infected with adenovirus Ad/pri-miR-21 (p<0.05); in contrast, a significantly decreased expression of miR-21 was observed in Ad/miR-21/inhibitor infected cells (p<0.05). Compared with pAd/con group, *: p<0.05. Results represented the mean ± SD from three independent triplicated experiments (N=9).