Figure 6

Suppression of invasion in vitro leads to suppression of metastasis in zebrafish following loss of JAK1 or JAK2 expression. Western blot analysis confirmed JAK-deficiencies in U4C and γ 2A cells (a). When human 2C4 fibrosarcoma cells are injected into zebrafish (b, left) (shown as z-stack images using 40× magnification), extensive spread throughout the body of the fish can be seen over 120 hpi. This metastasis potential correlates with in vitro invasion (b, right). In both JAK1-deficient U4C cells and JAK2-deficient γ2A cells, reduced levels of metastatic spread are seen (c, left), which correlates with reduced invasion in vitro (c, right).