EGF-induced phosphorylation of cofilin in human bladder cancer T24 cells were stimulated with EGF (50 ng/mL) for the indicated times (A), and with a range of EGF concentrations (1–100 ng/mL) for 30 sec (B). The cell lysates were immunoblotted with anti-phosphorylated ser-3 and anti-nonphosphorylated cofilin antibodies. (C) and (D) show statistical results obtained from the upper panels (A) and (B), respectively. The basal levels of cofilin phosphorylation are expressed as 100%. * denotes a statistically significantly difference from the basal levels of cofilin phosphorylation (p < 0.05).