Figure 4

Expression of ERGIC3 and cellular proliferation. The levels of ERGIC3 mRNA were evaluated by q-RT-PCR in GLC-82 cells after ERGIC3 gene silencing (A) and in BEAS-2B cells after ERGIC3 over-expression (B). And the levels of ERGIC3 protein were evaluated by western blot in GLC-82 cells after ERGIC3 gene silencing (C) and in BEAS-2B cells after ERGIC3 over-expression (D). Reduced expression of ERGIC3 could slow the proliferation rates of GLC-82 cells (E), but increased expression of ERGIC3 could enhance the proliferation rates of BEAS-2B cells (F). Differences in cell proliferation rates were analyzed by a paired t-test (two tailed). NC: negative control; ERGIC3i: ERGIC3 gene silencing; pLXSN: treated with the pLXSN vector; pLXSN-ERGIC3: treated with the pLXSN-ERGIC3 vector. *: P < 0.05; **: P < 0.01.