Table 2 Concordance between Luminex and direct sequencing
Gene | Direct sequencing (DS) | Luminex | Concordance rate | Mutation rate |
|---|---|---|---|---|
KRAS codon 61 | 3 | 3 | 100% | 3.6% |
Q61K | 0 | 0 | 100% | 0% |
Q61E | 0 | 0 | 100% | 0% |
Q61L | 0 | 0 | 100% | 0% |
Q61P | 0 | 0 | 100% | 0% |
Q61R | 0 | 0 | 100% | 0% |
Q61H | 3 | 3 | 100% | 3.6% |
KRAS codon 146 | 2 | 2 | 100% | 2.4% |
A146T | 2 | 2 | 100% | 2.4% |
A146S | 0 | 0 | 100% | 0% |
A146P | 0 | 0 | 100% | 0% |
A146E | 0 | 0 | 100% | 0% |
A146V | 0 | 0 | 100% | 0% |
A146G | 0 | 0 | 100% | 0% |
BRAF codon 600 | 4 | 4 | 100% | 4.9% |
V600E | 4 | 4 | 100% | 4.9% |
NRAS codon 12 | 2 | 2 | 100% | 2.4% |
G12S | 0 | 0 | 100% | 0% |
G12C | 0 | 0 | 100% | 0% |
G12R | 0 | 0 | 100% | 0% |
G12D | 2 | 2 | 100% | 2.4% |
G12V | 0 | 0 | 100% | 0% |
G12A | 0 | 0 | 100% | 0% |
NRAS codon 13 | 0 | 0 | 100% | 0% |
G13S | 0 | 0 | 100% | 0% |
G13C | 0 | 0 | 100% | 0% |
G13R | 0 | 0 | 100% | 0% |
G13D | 0 | 0 | 100% | 0% |
G13V | 0 | 0 | 100% | 0% |
G13A | 0 | 0 | 100% | 0% |
NRAS codon 61 | 0 | 0 | 100% | 0% |
Q61K | 0 | 0 | 100% | 0% |
Q61E | 0 | 0 | 100% | 0% |
Q61L | 0 | 0 | 100% | 0% |
Q61P | 0 | 0 | 100% | 0% |
Q61R | 0 | 0 | 100% | 0% |
Q61H | 0 | 0 | 100% | 0% |
PIK3CA Exon 9 | 1 | 1 | 100% | 1.2% |
E542K | 1 | 1 | 100% | 1.2% |
E545K | 0 | 0 | 100% | 0% |
E546K | 0 | 0 | 100% | 0% |
PIK3CA Exon 20 | 3 | 3 | 100% | 3.7% |
H1047R | 1 | 1 | 100% | 1.2% |
H1047L | 2 | 2 | 100% | 2.4% |