Figure 5

Pazopanib reduces vessel density in a xenograft orthotopic model of choriocarcinoma testicular germ cell tumor. (A) Staining for CD31 endothelial marker was carried out in viable zones of TGT38 choriocarcinoma tumors implanted in mice and treated as in Figure 4. Bar 40 μm, 400x. CD31 expression was analyzed by measuring the percentage of area with positive CD31 staining in a viable tumor area (B) and the number of vessel structures (C). Quantification was carried out using Image J software. The means and SDs of six sections of each tumor (four tumors per treatment group) of each variable are shown. *, p < 0.05 (two-tail Mann–Whitney U test).