Figure 3

pri- miR- 221/ 222 is strongly overexpressed in AML. pri-miR-221/222 levels were measured by qRT-PCR and normalized to those of the housekeeping gene ß-2-microglobulin using the ΔΔct method [40], with PB from healthy donor A as a calibrator in both panels. Please note that the scale of the y-axis differs between the panels. A) PB from 18 AML and 6 healthy controls (of which 3 and 4, respectively, had also been used in the experiment shown in Figure 2B; Additional file 1: Tables S1A-C); NPB, normal peripheral blood. B) BM from 19 AML (Additional file 1: Table S1C) and 8 healthy controls (Additional file 1: Table S1A; two controls had also been used for the experiment in Figure 2B); NBM, normal bone marrow. AML samples were enriched for leukemic blasts through Ficoll gradient purification. *, p < 0.05; **, p < 0.01.