Figure 2

Malignant human hematopoietic cells display impaired pri- miR- 221/ 222 processing capacity. Mature miR-221 was measured by qRT-PCR, normalized to RNU6B, and expressed relative to ß-2-microglobulin-normalized pri-miR-221/222. A) Human myeloid cell lines, and HeLa cells as a reference; B) primary AML samples (n = 8; Additional file 1: Table S1B), enriched for blasts through Ficoll gradient purification, and normal control PB (squares; n = 4) and BM (triangles; n = 2) samples (Additional file 1: Table S1A). HeLa cells were used as a calibrator in both experiments. *, p < 0.05; ***, p < 0.001.