Figure 2

Sensitivity of hepatocellular carcinoma (HCC) cells to 5-fluorouracil (5-FU) when treated in combination with sorafenib in vitro. Dose–response curves of 5-FU alone and combined with sorafenib, in different treatment sequences, are shown. HCC cell lines MHCC97H (A) and SMCC-7721 (B) were exposed to escalating doses of 5-FU, alone or combined with 8 μM sorafenib, in different treatment sequences. The cell survival fraction is expressed relative to the untreated cells, set at 100, and is expressed as the mean ± SD. Each value represents the average of three independent determinations with six replicates per experiment. Bars indicate standard error. S, sorafeinb; F, 5-FU.