Figure 10
From: Genetic and epigenetic analysis of putative breast cancer stem cell models
Association between DNA promoter methylation and mRNA expression of selected candidate genes in parental MCF7 and SUM159 cells and their corresponding CSC phenotype. DNA methylation was assessed by pyrosequencing and mRNA expression by qRT-PCR. Bars represent average and SD of two biological replicates. *p < 0.05. (A) DNA promoter methylation and (B) mRNA expression levels in parental and MMO MCF7 cells. (C) DNA promoter methylation and (D) mRNA expression levels in parental and MMO SUM159 cells. (E) DNA promoter methylation and (F) mRNA expression levels in sorted subpopulations of SUM159 cells.