Figure 1

The expression of YAP 1 in UCB and normal bladder tissues. (A) Up-regulated expression of YAP 1 mRNA was examined by qRT-PCR in 12/14 UCB cases, when compared with paired normal bladder tissues. Expression levels were normalized for β-actin. Error bars, SD calculated from three parallel experiments. (B) Up-regulated expression of YAP 1 protein was detected by Western blotting in 11/14 UCB cases, when compared with paired normal bladder tissues. Expression levels were normalized with GAPDH. (C-F) The expression of YAP 1 in UCB and normal bladder tissues by IHC (100×). An UCB (case 39) tissue showed high expression of YAP 1, in which more than 90% of tumor cells were positively stained by YAP 1 in the nucleus (C), while its paired normal bladder urothelial mucosal tissue was negatively stained by YAP 1 (D). High expression of YAP 1 was observed in another UCB tissue (case 102), in which about 70% of tumor cells demonstrated a nuclear staining with a lesser cytoplasmic staining of YAP 1 (E). An UCB (case 78) was examined low expression of YAP 1, in which less than 5% of tumor cells showed nuclear staining of YAP 1 (F). An UCB (case 114) tissue showed high expression of YAP 1, in which more than 90% of tumor cells were positively stained by YAP 1 in the cytoplasm (G).