Figure 4
ATRA does not affect CysLT 2 R in HCT-116 cells. RAR-mutated HCT-116 colon cancer cells were incubated for 3, 12, or 24 h in the presence or absence of 1 μM ATRA. (A) qPCR for CysLT2R expression in HCT-116 cells. Data are normalized to HPRT-1. (B) Protein expression of CysLT2R normalized to β-actin in HCT-116 cells as determined by Western blot. Data show changes in expression relative to basal levels in unstimulated cells. (n.s. = not significant).