Figure 2
Effect of RARα or RARβ inhibition on CysLT 2 R expression and promoter activity. SW480 cells were transfected with control siRNA (non-target siRNA), RARα siRNA, or/and RARβ siRNA, allowed to rest for 24 h, serum-starved overnight, and subsequently stimulated with 10 μM ATRA. (A and B) qPCR for CysLT2R and RARα expression. Data are normalized to HPRT-1 with and without ATRA stimulation for 3 h. (C) Western blots without and with ATRA stimulation for 3 h. (D) Western blot analyzes of CysLT2R protein expression in membrane fractions from SW480 cells stimulated or not with ATRA for 3 h normalized to unstimulated control. (E) CysLT2R luciferase promoter activity in cells stimulated with or without ATRA for 48 h. Data are normalized to unstimulated controls. (F) CysLT2R luciferase promoter activity in siRNA-treated cells stimulated with or without ATRA for 48 h. Data are normalized to unstimulated control. Values are shown as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).