The effect of HWF on cell scattering of HN-7 cells. (a) At time 0 the medium was changed to DMEM containing 10% FBS, HS, or HWF as indicated. The cells were photographed with a 10× objective at the specified time points. (b) Photographs sampled as under A were analyzed in ImageJ software as stated in Materials and Methods to determine the area covered by cell colonies (N=16). (c) The exponential area increase in the later time points in panel B was determined by non-linear regression and subtracted from the total area increase, yielding an approximation of the scatter contribution (N=16). (d) As in panel B, cells were exposed to 10% HWF at time 0, but after approximately 4 h the medium was changed back to 10% FBS (N=8). (e) The cells were exposed to 10% HWF collected at different time points after operation (OP). The area increase during the first hour was determined as in panel B (N=8). Error bars represent SEM.