Figure 2

The suppressive effect of Sox2 on the invasiveness in RU subset but not RR subset. (A) Cell invasiveness was also assessed using RR cells, RU cells and unsorted cells (labeled as 'Sox2R') derived from MCF7. No significant difference in invasiveness was observed between these three cell populations. Triplicate experiments were performed. A representative experiment is shown (mean ± standard deviation) (n = 3). (B) MCF7-RR and -RU cells were subjected to either scramble siRNA or Sox2 siRNA treatment for 24 hour before invasion assay. Sox2 siRNA treatment resulted in significant increase in invasiveness in MCF7-RU cells; no significant change was observed in MCF7-RR cells. Triplicate experiments were performed. A representative experiment is shown (mean ± standard deviation) (n = 3). (C) Cell viability of RR, RU, and unsorted cells (labeled as 'Sox2R) from MCF7 were assessed by the MTS assay. (D) MCF7-RR and -RU cells were treated with Sox2 siRNA or scramble siRNA before the MTS assay. No significant change in cell viability was found after Sox2 siRNA treatment. (E) ZR751-RU cells were subjected to either scramble siRNA or Sox2 siRNA treatment for 24 hour before invasion assay. Sox2 siRNA treatment significantly increases the invasiveness in ZR751-RU cells. Cell viability assay was also performed and no significant change was observed after Sox2 siRNA treatment.