Figure 6

Tumor xenograft. (A) Tumor formation was monitored twice-weekly over a 43-day period in five groups of mice that received subcutaneous injections of A549 cells, which express dnhWnt-2 or vector control expression constructs (mean ± standard deviation, p = 0.007). (B) Tumor mass was measured on day 43 (mean ± standard deviation, p = 0.02). (C) Immunohistochemical analysis of cell proliferation by Ki-67 staining was performed in tumor sections expressing vector control (left panel) or the dnhWnt-2 (right panel) at 40× magnification. Inset is at 10× magnification. (D) qRT-PCR analysis of Wnt downstream target genes Survivin, c-Myc, DVL3, and Cyclin D1 in A549 xenograft tumors with either dnhWnt-2 or vector control. Expression of GAPDH was used as a control transcript.