Figure 3
Karyotype analysis of genomic instability in bone marrow cells of mouse after exposure to RAN extract with (RAN + L) or without lime (RAN). (A) Percentage of metaphases with premature sister-chromatid separation. Two mice per point for untreated, 15 and 30 days of treated group and three mice per point for the rest. At least 100 metaphases were scored to each mouse. (B) Normal metaphase spread from mouse bone marrow cells. (C) Premature sister-chromatid separation from mouse exposed to RAN. Brackets show sister-chromatids lying separated in mitotic figures that show the phenotype. (D and E) Metaphase spread showed 37 and 38 chromosomes, respectively. (F,G and H) Metaphase spread showed more than 60 chromosomes. (I) showed more than 80 chromosomes.