Expression of p65 in epidermal tumors and normal skin by 96 IHC EnVision (magnification × 400). (a) to (g). Positive expression of p65 was located in the cytoplasm, cell, and (or) nucleus after activation. The black arrow shows expression of p65 in the epithelial nucleus, the orange arrow shows p65 expression in the epithelial cytoplasm, the blue arrow shows p65 expression in an inflammatory cell and the green arrow shows p65 expression in a vascular endothelial cell. (h). **P < 0.01 as compared with p65 in the epithelial nuclei in different groups (i). **P < 0.01 as compared p65 in inflammatory cell nuclei in SK. The error bars represent the standard error of the mean (SEM). (j). Western blot detection of p65 expression in epithelial nuclei, which increased gradually from NS, SK, PCL, BCC, and to SCC. Histone H3 was used as a loading control.