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Table 2 The effect of vitamin D analog PRI-2205 (in varied doses) alone or in combination with 5-FU on tumor growth, life span and body weight of mice bearing subcutaneous colon cancer MC38

From: Vitamin D analogs enhance the anticancer activity of 5-fluorouracil in an in vivomouse colon cancer model

No. 5-FU PRI-2205 TGI [%] ILS [%] Maximal decrease
in body weight [%]
5-FU PRI-2205 5-FU+ %H TGI Effect 5-FU PRI-2205 5-FU+ %H ILS Effect 5-FU PRI-2205 5-FU+
PRI-2205 PRI-2205 PRI-2205
1 75 mg/kg; 10 μg/kg 48 7 76* 44 Synergism 21 2 77*a 23 Synergism 7 10 8
2 50 mg/kg; 10 μg/kg 30 -16 70 19 Synergism 12 0 38* 12 Synergism 0 0 0
3 150 mg/kg; 10 μg/kg 87 25 96* 90 Synergism nt. nt. nt. nt. nt. 9 1 19
4 100 mg/kg; 5 μg/kg 31 -12 26 23 Antagonism 26 -6 12 22 Antagonism 4 1 6
5 100 mg/kg; 2.5 μg/kg 31 -41 19 2 Antagonism 26 -15 32 16 Synergism 4 7 4
  1. Vitamin D analog was administered subcutaneously, three times a week.
  2. No. – number of experiment; TGI – tumor growth inhibition; %H TGI: hypothetical tumor growth inhibition; ILS: increase in life span; %H ILS: hypothetical increase in life span; nt.: not tested; No. 1 – TGI calculated on the 27th day of the experiment, No. 2, 4, 5 - TGI on the 30th day of the experiment; No. 3 – TGI on the 32nd day of the experiment.
  3. No. 1: 5-FU was injected i.p. on the first day; PRI-2205 was injected on days: 1, 3, 6, 8, 10, 13, 15, 17, 20, 22; Number of mice: control - 10; in the each treatment group - 8.
  4. No. 2: 5-FU was injected i.p. on days: 2, 7, 11, 16; PRI-2205 was injected on days: 2, 4, 7, 9, 11, 14, 16, 18, 21; Number of mice: control – 9, 5-FU – 8, 5-FU + PRI-2205 – 8, PRI-2205 – 7.
  5. No. 3: 5-FU was injected i.p. on days: 9, 16, 23; PRI-2205 was injected on days: 11, 14, 16, 18, 21, 23, 25, 28; Number of mice in each group - 8.
  6. No. 4 and 5: 5-FU was injected i.v. on days: 8, 15, 30, 38; PRI-2205 on days: 9, 11, 14, 16, 18, 21, 23, 25, 28, 30, 32, 35, 37, 39, 42, 44; Number of mice: No. 4 and 5: control – 7, 5-FU – 7, PRI-2205 – 5, 5-FU + PRI-2205 – 6; * P < 0.05 as compared to control; *a P < 0.05 as compared to 5-FU.