Figure 5

Histological section of mice for evaluation of tumor seeding. (A and B) Representative longitudinal sections of the brains and spinal cords of the mice with D283-control-shRNA or D283-ID3-shRNA (hematoxylin & eosin stain) (1.25×). Inlet figures denote (a) subfrontal area, (b) craniocervical junction (cistern magna) where the tumor cells were first implanted, (c) upper thoracic spinal cord, (d) midthoracic spinal cord, (e) lumbar spinal cord, and (f) conus medullaris (100×). (C and F) Immunofluorescence (IF) staining with anti-Ki-67, a proliferation marker, on the specimen. (D and G) IF staining with anti-caspase-3, an apoptosis marker. Scale bars represent100 μm. (E and H) IF staining with anti-ID3. (I and J) Comparison of the proportions of Ki-67⁺ cells and caspase-3⁺ cells.