Figure 5
Phosphoprotein and total protein expression profiles of MCF7 (left) and MDA-MB-231 (right) breast cancer cells treated with TAM and/or CXB. Phosphorylated levels of p-Src (Tyr416), p-STAT3 (Tyr705), p-Akt (Ser473), p-MAPK (Thr202/Tyr204) and p-Bad (Ser136) were determined by western blot analysis using their specific antibodies. β-Actin was used as an invariant control for equal loading.