Figure 3
From: Claudin 1 expression in basal-like breast cancer is related to patient age
Subcellular localization of claudin 1 protein in BT-20 cells. Subcellular fractions of control BT-20 cells were analyzed by Western blot using the claudin 1 antibody. A. Short exposure shows claudin 1 in the membrane fraction only, B. longer exposure reveals some protein in the cytoskeletal and to a lesser extent, the nuclear fraction. The arrow indicates the 21kD claudin 1 protein. C. Immunofluorescent staining with the claudin 1 antibody (left panels) shows positive fluorescence for claudin 1 in the cell membrane and the cytoplasm of a control clonal cell line and reduced fluorescence in the claudin 1 knockdown clone (clone 3).