Figure 2
From: OSU-03012 sensitizes breast cancers to lapatinib-induced cell killing: a role for Nck1 but not Nck2
ER stress via PERK activation may be responsible for lapatinib/OSU-03012-induced cytotoxicity/cytostaticity. A-B: MDA-MB-231 cells, 24 h after plating, were transfected with the indicated siRNA. After a 24 h incubation, cells were either plated singly onto 6-well plates and allowed to attach overnight (A) or harvested for immunoblotting to ensure knockdown (B). Cells in (A) were treated with vehicle or OSU-03012/lapatinib (48 h) then media was replaced and colonies were allowed to develop over the next 10-14 d. Colonies were counted using crystal violet stain and the number of colonies was graphed (n=3, *=p<0.05).