Effect of miR-34a on choriocarcinoma cells. (A) Cell proliferation upon miR-34a ectopic expression. Significantly slower proliferation was observed in cells with miR-34a ectopic expression at 168 hours post-transfection. (B) Colony formation of pre-miR-34a transfected cells seeded at low density. The colonies were visualized after staining with crystal violet at 14-days post-transfection. The bars in the chart represent mean ± SD of number of colonies from 3 independent experiments. * p < 0.05. (C) Suppression of invasion of BeWo and JEG-3 cells upon miR-34a ectopic expression. Representative images of the invaded cells. The graph represents the extent of invasion of the pre-miR-34a transfected cells relative to the control cells.