Figure 6

Effect of equol and 4-OHT on bcl-2 and bax expression. MCF-7 were treated for 48 and 72 h with equol (100 μM) and 4-OHT (10 μM) and whole cell extracts were prepared. Protein expression was then analyzed by western blot using anti-bcl-2 and anti-bax polyclonal antibodies. Data are representative of three individual experiments. C, vehicle control; E, Equol (100 μM); T, 4-OHT (10 μM), E + T, Equol (100 μM) + 4-OHT (10 μM).