Figure 1

Post-translational regulation of PTEN by cisplatin treatment. (A) PTEN protein levels in A2780 cells after 24 hrs cisplatin treatment. GAPDH was used as loading control. Graphs show densitometric analysis of three independent experiments. * p < 0.05 compared to vehicle treated cells. (B) Real-time quantitative PCR analysis for PTEN relative mRNA levels in A2780 cells after 24 hrs of cisplatin treatment. GAPDH was used as internal control. (C &D) A2780 cells were grown on glass coverslips and treated with cisplatin for 24hrs. Following treatment, cells were fixed using 4% paraformaldehyde and immunofluorescence was performed using PTEN (Green) antibody. Nuclei were counterstained with Hoechst 33258 (blue) and rodamine phalloidin (red) was used to stain actin cytoskeleton and to visualize the shape and cellular integrity. Magnification: 63 × .