Figure 1

Genetic findings in unfavorable neuroblastoma. The frequency of copy number changes was calculated for all measurement points in the arrays and plotted relative to the position along the chromosome for: (A): all tumors, (B): MNA^not11q- tumors, (C): 11q-^notMNA tumors, (D): neither MNA nor 11q loss tumors. The number of analyzed tumors is indicated (n). Green bars above the horizontal line indicate the percentage of tumors with copy gains and red bars below the horizontal line indicate the percentage of tumors with copy losses. Data for the X chromosome were normalized to female reference DNA and the respective proportion of boys in panels A-D were: 56%, 43%, 67%, and 67%, respectively (E): To search for copy number alterations that differ between the11q-^notMNA and MNA^not11q- groups, the frequency percentage difference between the two groups are plotted: Copy number gain difference (green graph): Values above baseline represent regions in which gains are more numerous among 11q-^{not MNA} tumors, and vice versa for values below baseline. Deletion difference (red graph): Values above baseline represent regions in which losses are more common among MNA^not11q- tumors, and vice versa for values below baseline. The regions significantly differentially altered between the groups, identified by using Fisher's exact test within Nexus copy-number software, (p<0.05 and threshold difference in frequency >25%), are shown below the graph, as indicated by a black arrow.