Figure 1

Wnt pathway deregulation in murine and human NSCLC. Comprehensive gene expression microarrays and qPCR assays reveal deregulation of specific components of the Wnt signaling pathway in murine and human NSCLC. (A)161 probes on the array representing 117 unique genes defined by Gene Ontology under the classification Wnt receptor signaling pathway are significantly (ANOVA, P ≤ 0.05) over- or under-expressed in murine cyclin-E transgenic lung cancers as compared to adjacent normal or non-transgenic mouse lung. (B) The qPCR-based assays of Wif1 expression levels in a panel of paired malignant (samples labeled T) and normal (samples labeled N) lung tissues from 12 transgenic cyclin E mice, both wild type (WT) and proteasome-degradation resistant (T62A/T380A). (C) The qPCR-based measurements of TNKS1 and TNKS2 expression levels in the same panel of paired malignant and normal murine lung tissues. (D) The qPCR-based measurement of Wif1 (left panel) and TNKS1 and TNKS2 (right panel) expression levels in three human lung adenocarcinoma tumor samples versus adjacent normal lung tissue.