Table 2 External reproducibility panel design

1

Wild Type

2

Exon 19 – deletion mutation #1 – LOD

(EX19_ 2235_2249del15 - 5% Mutation)

3

Exon 19 – deletion mutation #2 – LOD

(EX19_2236_2250del15 - 5% Mutation)

4

Exon 19 – deletion mutation #3 - LOD

(EX19_2239_2248 > C - 5% Mutation)

5

Exon 19 – deletion mutation #4 - LOD

(EX19_2240_2254del15 - 5% Mutation)

6

Exon 19 – deletion mutation #5 - LOD

(EX19_2240_2257del18 - 5% Mutation)

7

Exon 21 L858R mutation – LOD

(EX21_ 2573T > G = L858R - 5%Mutation)

8

Exon 19 – deletion mutation #1 –2 × LOD

(EX19_ 2235_2249del15 - ≤10% Mutation)

9

Exon 19 – deletion mutation #2 –2 × LOD

(EX19_2236_2250del15 - ≤10% Mutation)

10

Exon 19 – deletion mutation #3 –2 × LOD

(EX19_2239_2248 > C - ≤10% Mutation)

11

Exon 19 – deletion mutation #4 –2 × LOD

(EX19_2240_2254del15 - ≤10% Mutation)

12

Exon 19 – deletion mutation #5 –2 × LOD

(EX19_2240_2257del18 - ≤10% Mutation)

13

Exon 21– L858R mutation –2 × LOD

(EX21_ 2573T > G = L858R -≤10% Mutation)